大家都在搜
手机验证
发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Rat
96T
货号 | EK0406 |
---|---|
产品名称 | Rat IL-4 ELISA Kit |
产品描述 | For quantitative detection of rat IL-4 in cell culture supernates and serum. |
免疫原 | E.coli, C25-S147 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <1pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | IL4 |
基因全名 | interleukin 4 |
Uniprot ID | P20096 |
指标别名 | B cell growth factor 1|B cell stimulatory factor 1|BCGF 1|BCGF1|Binetrakin|BSF 1|BSF1|IL 4|Interleukin 4|Lymphocyte stimulatory factor 1|MGC79402|Pitrakinra |
背景资料 | Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0406-CAP | 96-well plate precoated with anti- rat IL-4 antibody | 1 |
EK0406-ST | Lyophilized recombinant rat IL-4 standard | 10ng/tubex2 |
EK0406-DA | Biotinylated anti- rat IL-4 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 35 | 164 | 456 | 32 | 175 | 481 |
Standard deviation | 2.45 | 10.66 | 21.43 | 2.4 | 11.37 | 28.37 |
CV(%) | 7% | 6.5% | 4.7% | 7.5% | 6.5% | 5.9% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.057 | 0.106 | 0.159 | 0.263 | 0.517 | 1.043 | 1.781 | 2.383 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。