培养基:The base medium for this cell line is DMEM:F12 Medium (ATCC 30-2006).To make the complete growth medium, add the following components to the base medium:
extra 10 mM HEPES (for a final conc. of 25 mM)
10 ng/ml cholera toxin
0.005 mg/ml insulin
0.005 mg/ml transferrin
100 ng/ml hydrocortisone
20 ng/mL human recombinant EGF (Thermo Fisher PHG0311)
Fetal Bovine Serum, 10% final conc (ATCC 30-2020)
传代比例:1:2 -1:4