SYBR™ Select Master Mix 现货供应 货号4472908 描述
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Alternative Product: Try
PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Select Master Mix and added additional capabilities for your gene expression analysis.
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SYBR® Select Master Mix is a superior and cost-effective solution for your real-time PCR applications.
SYBR™ Select Master Mix 现货供应Features of the SYBR® Select Master Mix include:• Specific—minimize primer-dimer and non-specific amplification
• Reproducible and sensitive—consistent amplification across a wide dynamic range
• Bright—contains SYBR® GreenER™ dye for maximum brightness
• Carry-over contamination control—contains heat-labile UDG
• Can be used in either standard or fast cycling mode
Formulated for Maximum SpecificitySYBR® Select Master Mix contains all the components needed for your real-time PCR reaction, except the template and primers, in a convenient 2X concentration premix. The master mix includes AmpliTaq® DNA Polymerase, UP, a highly purified DNA polymerase with a proprietary hot start mechanism that provides exceptional specificity.
Obtain Reproducible Results Across a Wide Dynamic RangeSYBR® Select Master Mix is specially formulated to provide robust results from 100 ng to 0.1 pg cDNA per reaction (Figure 1) and can reliably detect a single copy of genomic DNA (Figure 2).
Contains SYBR® GreenER™ Dye and Heat-labile UDG• SYBR® GreenER™ dye is less inhibitory to PCR than SYBR® Green I dye resulting in brighter signals
• Heat-labile uracil-DNA glycosylase (UDG) is included for worry-free carryover contamination control
SYBR™ Select Master Mix 现货供应Instrument CompatibilitySYBR® Select Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems® real-time PCR instruments except the 7900HT real-time PCR system. It is also compatible with the Bio-Rad IQ™5, Roche LightCycler® LC480, and Stratagene® MX3005P® systems.
规格
| Detection Method: | SYBR |
| For Use With (Equipment): | 7500 Fast System, 7500 System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Fast Mode, StepOne™, Standard Mode, StepOnePlus™, Fast Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Reaction Speed: | Fast, Standard |
| PCR Method: | qPCR |
| Sample Type (General): | DNA |
| Form: | Liquid |
| Format: | Vial(s) |
| Hot Start: | Built-In Hot Start |
| Label or Dye: | SYBR® GreenER™ |
| Passive Reference Dye: | ROX (Pre-mixed) |
| Polymerase: | AmpliTaq® DNA Polymerase |
| Primer-Probe Compatibility: | Unlabeled Oligos |
| Product Line: | SYBR® |
| Product Size: | 1 x 5 mL |
| Purity or Quality Grade: | UP (Ultra Pure) |
| Sample Type (Specific): | DNA (Genomic), cDNA |
| Shipping Condition: | Wet Ice |
内容及储存SYBR™ Select Master Mix 现货供应
The 2x mix contains SYBR® GreenER™ dye, AmpliTaq® DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 µL reactions (10 µL Master Mix per reaction). Store at 2–8°C.
Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.ABI相关产品
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