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KLH conjugated
IgG
冻干粉
负20°保存
多克隆
详情请来电索取说明书
详情请来电索取说明书
Rabbit
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
1mg/ml
详情请来电索取说明书
TIMP-1
金属蛋白酶组织抑制因子1
20ul/50ul/100ul/200ul
规格: | 20ul | 产品价格: | ¥380.0 |
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规格: | 50ul | 产品价格: | ¥780.0 |
规格: | 100ul | 产品价格: | ¥1380.0 |
规格: | 200ul | 产品价格: | ¥2200.0 |
产品图片 | Sample: Lane1: Brain(Mouse) Lysate at 30 ug Lane2: Liver (Mouse) Lysate at 30 ug Primary: Anti-TIMP-1 (bs-0415R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 21kD Observed band size : 26kD Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(bs-0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(bs-0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(bs-0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rabbit liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(bs-0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
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