Anti-ASCT2
Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2, SLC1A5
Cat #: ANT-082
Sizes: 25 µl, 50 µl, 0.2 ml
Source: Rabbit
Type: Polyclonal
Applications: IH, WB
May also work in: IC, IFC, IP
Reactivity: M, R
Application key:
CBE- Cell-based ELISA, FC- Flow cytometry, IC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IH- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot
Species reactivity key:
H- Human, M- Mouse, R- Rat
CLICK HERE TO RECEIVE A 25 µl FREE TRIAL SAMPLE!
Anti-ASCT2 (#ANT-082) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC1A5 from mouse and rat samples. Will not recognize human ASCT2.
Western blot
Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4):
1, 2. Anti-ASCT2 antibody (#ANT-082), (1:200).
3, 4. Anti-ASCT2 antibody, preincubated with the control peptide antigen.
Immunohistochemistry
Expression of ASCT2 in mouse hippocampus
Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASCT2 antibody (#ANT-082), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASCT2 staining appears in a layer (arrows) adjacent to the dentate granule layer (GL). Cell nuclei are stained with DAPI (blue).
Immunogen
Peptide (C)KMPSSEPELIQVKNE, corresponding to amino acid residues 502 - 516 of mouse ASCT2 (Accession P51912). Intracellular, C-terminus.
HomologyRat – 14/15 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
SpecificityWill not recognize ASCT2 from human samples.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionThe antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl double distilled water (DDW).
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background
ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.
ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.
Related products
- Anti-ASCT1 (#ANT-081)
- Anti-Cystine/Glutamate Transporter (xCT) (extracellular) (#ANT-111)
- Anti-Cationic Amino Acid Transporter 2 (extracellular) (#ANT-103)
- Anti-Glucose Transporter 1 (GLUT1) (#AGT-021)
- Anti-Glucose Transporter 3 (GLUT3) (extracellular) (#AGT-023)
- Anti-Serotonin Transporter (SERT) (extracellular) (#AMT-004)