手机验证
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液体或冻干粉
-20°C
多克隆
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6个月
Rabbit
AZT-008
高
大量
上海信裕生物科技有限公司
Rabbit
WB, IC*, IFC*, IH*, IP*, LCI*
见官方网站
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Anti-Zinc Transporter ZIP8 (extracellular)
Anti-Zinc Transporter ZIP8 (extracellular)
25 µl, 50 µl, 0.2 ml
Anti-Zinc Transporter ZIP8 (extracellular) (#AZT-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize ZIP8 from human, rat, and mouse samples.
Western blot
Western blot analysis of mouse kidney lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
1, 2. Anti-Zinc Transporter ZIP8 (extracellular) antibody (#AZT-008), (1:200).
3, 4. Anti-Zinc Transporter ZIP8 (extracellular) antibody, preincubated with the control peptide antigen.
Western blot analysis of rat brain lysate:
1. Anti-Zinc Transporter ZIP8 (extracellular) antibody (#AZT-008), (1:200).
2. Anti-Zinc Transporter ZIP8 (extracellular) antibody, preincubated with the control peptide antigen.
Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human K562 chronic myelogenous leukemia cell line lysate (lanes 2 and 4):
1, 2. Anti-Zinc Transporter ZIP8 (extracellular) antibody (#AZT-008), (1:200).
3, 4. Anti-Zinc Transporter ZIP8 (extracellular) antibody, preincubated with the control peptide antigen.
Immunogen
Peptide (C)KTYGQNDHTHFRND, corresponding to amino acid residues 221-234 of rat ZIP8 (Accession Q5FVQ0). 2nd extracellular loop.
HomologyMouse – identical; human – 12/14 amino acid residues identical.
PurityAffinity purified on immobilized antigen.
FormulationLyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Standard quality control of each lotWestern blot analysis.
Peptide confirmationConfirmed by amino acid analysis and mass spectrometry.
Storage before reconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Reconstitution25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution0.8 mg/ml.
Storage after reconstitutionThe reconstituted solution can be stored at 4°C for up to 2 weeks. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Control antigen storage before reconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control antigen reconstitution100 µl DDW.
Control antigen storage after reconstitution-20ºC.
Preadsorption Control1 µg peptide per 1 µg antibody.
Scientific background
Zinc uptake transporter, ZIP8, belongs to the SLC39 transporter family. ZIP transporters are responsible for zinc transport into the cytoplasm across cellular membranes, either influx from the extracellular space or efflux from intracellular organelles. Regulation and maintenance of proper concentration and distribution of cellular zinc nutrient by ZIP transporters are essential to the function, metabolism, growth, proliferation and survival of cells1,2.
ZIP structure contains eight predicted transmembrane (TM) domains while the N- and C-termini are extracellular. A long loop region is present between TM3 and TM4 domains, harboring a histidine-rich region. TM4 and TM5 domains are amphipathic and are thought to form a cavity through which metals are transported1.
ZIP8 protein is encoded by the SLC39A8 gene and is highly expressed in T cells. ZIP8 also plays an important role at the onset of inflammation2.
Zinc deficiency can cause growth retardation, cognitive impairment, metabolic disorders, infertility, dysfunction of the immune system, abnormalities in T-cells, natural killer cells and monocytes, and reduction in antibody formation1,2. Studies exploring knockdown of ZIP8 protein show a reduction of cellular zinc content, impaired mitochondrial function in response to TNF-α and increased cell death2.
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