HMGB1高迁移率族蛋白B1抗

体

HMGB1高迁移率族蛋白B1抗

体

¥1380

价格 ¥1380
品牌雅吉
产地中国
货号 s-0664R
更新日期 2023年10月25日

上海雅吉生物科技有限公司

品牌商

8 年皇冠会员

免疫原：
KLH conjugated synthetic（具体详情咨询我们）
亚型：
IgG
形态：
Lyophilized or Liquid
保存条件：
Store at -20 °C
克隆性：
多克隆
标记物：
有各种标记抗体需要可以联系我公司
适应物种：
Hu Mo Pig Cow Hor Sheep等
宿主：
Rabbit
应用范围：
WB=1:100-500,Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,Flow Cyt=1μg |test,IF=1:100-500,ICC=1:100-506,
浓度：
1mg/ml
靶点：
详询
抗体英文名：
HMGB1
抗体名：
高迁移率族蛋白B1抗体
规格：
100ul

高迁移率族蛋白B1抗体

产品图片

Sample: bone (mouse) Lysate at 40 ug
Primary: Anti- HMGB1(bs-0664R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 25 kD
Observed band size: 27kD

Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGB1 Polyclonal Antibody, Unconjugated(bs-0664R) 1:200, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HMGB1 Polyclonal Antibody, Unconjugated(bs-0664R) 1:400, overnight at 4癈, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-HMGB1 antibody (bs-0664R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Rat splenocytes stained with Anti- HMGB1 Polyclonal Antibody, A488 Conjugated (bs-0664R-A488) at 1:50.

The figure annotation:
The blue histogram is unstained cells. The Orange histogram is cells stained with Rabbit IgG/FITC (bs-0295P-FITC) The green histogram is cells stained with Rabbit Anti-HMGB1/FITC Conjugated antibody (bs-0664R-FITC).
Controls
Positive control: HepG 2 cells Isotype control: Cell lines treated with Rabbit IgG/FITC (bs-0295P-FITC). instead of the primary antibody to confirm that primary antibody binding is specific. 5μgin 100 μL 1 X PBS containing 0.5% BSA.