This product is purified human IgE with kappa light chains produced in vitro from a monoclonal hybridoma. Original material is obtained from a healthy donor tested negative by US-FDA approved tests against HIV, HCV and Hepatitis B.Must be handled as potentially infectious as all human material.The purity of the human IgE is > 90%, tested by SDS-PAGE.As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The human IgE is purified by Protein L chromatography. The remaining contaminants are mainly components from Foetal Bovine Serum.
Immunogen
Non-immune
Subclass
IgE/k
Epitope
Non-immune
Content
100 µl, 1mg/mL
Purification
Protein L purified, Biotinylated
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, with 0.15 M NaCl and 0.09 % azide
Storage
Store at 2 to 8ºC. Do not freeze
Application 1
Quantifying Assay This antibody is well suited as a standard in IgE quantifying assays due to its very low batch-to-batch variation. Other applications include immunochemistry and cellular immunology research.
Application 2
IHC This antibody can be used in immunochemistry
Application 3
Cell Stimulation Asssay
This antibody can be used in cellular immunology research