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-20℃
6个月
pMHE2
大量
钰博生物
1ug/5ug
出品人: | Ybscience |
---|---|
载体名称: | pMHE2 |
质粒类型: | 广宿主载体;细菌表达载体 |
高拷贝/低拷贝: | -- |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | T7/lac |
载体大小: | 5878 bp |
5' 测序引物及序列: | T7 promoter 5’TAATACGACTCACTATAGGG 3’ |
3' 测序引物及序列: | T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’ |
载体标签: | 6XHis tag (N ter)、EK site |
载体抗性: | 链霉素 |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 多个菌属 |
备注: | -- |
组成型/诱导型: | 诱导型 |
LOCUS AY299693 5878 bp DNA circular SYN 10-FEB-2004DEFINITION Broad host range expression vector pMHE2, complete sequence.ACCESSION AY299693VERSION AY299693.1 GI:34334140REFERENCE 1 (bases 1 to 5878) TITLE Modular broad-host-range expression vectors for single-protein and protein complex purification JOURNAL Appl. Environ. Microbiol. 70 (2), 712-721 (2004) PUBMED 14766546 AUTHORS Fodor,B.D. TITLE Direct Submission JOURNAL Submitted (16-MAY-2003) Biophysics, BRC Hungarian Academy of Sciences, Temesvari krt., Szeged, Csongrad 6701, HungaryFEATURES Location/Qualifiers source 1..5878 /organism="Broad host range expression vector pMHE2" /mol_type="genomic DNA" /db_xref="taxon:244051" primer_bind 207..225 /note="T7 promoter primer" regulatory 207..223 /regulatory_class="promoter" /note="T7 promoter" misc_feature 224..249 /note="lac operator" regulatory 280..286 /regulatory_class="ribosome_binding_site" misc_feature 294..296 /note="fusion protein start codon" misc_feature 300..317 /note="His-tag" misc_feature 318..329 /note="Factor Xa cleavage site" primer_bind complement(428..445) /note="T7 terminator primer" regulatory 441..488 /regulatory_class="terminator" /note="T7 terminator" rep_origin 1120..1782 /note="pBBR1 oriV" misc_feature 2776..3769 /note="RP4 mob; oriT" regulatory complement(3859..3974) /regulatory_class="terminator" /note="T4 terminator" gene complement(4234..5244) /gene="aadA" CDS complement(4234..5244) /gene="aadA" /note="streptomycin resistance" /codon_start=1 /transl_table=11 /product="aminoglycoside-3:adenyltransferase fusion" /protein_id="AAQ64664.1" /db_xref="GI:34334141" /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA PKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV GK" regulatory 5761..5876 /regulatory_class="terminator" /note="T4 terminator"ORIGIN 1 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac 61 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat 121 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga 181 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga 241 taacaattcc cctctagaaa taattttgtt taactttaag aaggagatat accatggggc 301 atcatcatca tcatcatatc gagggaaggc ctgtcgacgg tatcgataag cttgatatcg 361 aattcctgca gcccggggga tccggctgct aacaaagccc gaaaggaagc tgagttggct 421 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 481 ggttttttgc tgaaaggagg aactatatcc ggatcgccct tcccaacagt tgcgcagcct 541 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 601 taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg cggggcgtaa 661 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata 721 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca 781 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag 841 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga 901 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa 961 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg 1021 cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc 1081 tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac 1141 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg 1201 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg 1261 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg 1321 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc 1381 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc 1441 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc 1501 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac 1561 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt 1621 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc 1681 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt 1741 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta 1801 gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc agcgcgacaa 1861 caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc tacgcaatga 1921 gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa gttgttgatt 1981 tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga agggcacccc 2041 tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg 2101 ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa 2161 cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc 2221 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg 2281 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc 2341 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg 2401 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct 2461 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac 2521 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg 2581 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc 2641 cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca 2701 ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa 2761 aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg 2821 ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac 2881 gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc 2941 cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac 3001 gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc 3061 ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc 3121 agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag 3181 gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag 3241 accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag 3301 gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac 3361 gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa 3421 aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa 3481 gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc 3541 aagaaggccg atcaagccca agagacggcc cgagaccttc gggagcgcct gaagcccgtt 3601 ctggacgccc tggggccgtt gaatcgggat atgcaggcca aggccgccgc gatcatcaag 3661 gccgtgggcg aaaagctgct gacggaacag cgggaagtcc agcgccagaa acaggcccag 3721 cgccagcagg aacgcgggcg cgcacatttc cccgaaaagt gccacctggc ggcgttgtga 3781 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt 3841 ggcggtactt gggtcgatag ctttatgctt gtaaaccgtt ttgtgaaaaa atttttaaaa 3901 taaaaaaggg gacctctagg gtccccaatt aattagtaat ataatctatt aaaggtcatt 3961 caaaaggtca tccaccggat cagcttagta aagccctcgc tagattttaa tgcggatgtt 4021 gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat gatatatctc 4081 ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac ttgacctgat 4141 agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa gccgcgccgc 4201 gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc 4261 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc 4321 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag 4381 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct 4441 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg 4501 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg 4561 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt 4621 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc 4681 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat 4741 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga 4801 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac 4861 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga 4921 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac 4981 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactttgt 5041 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc 5101 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca 5161 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt 5221 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc 5281 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt 5341 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt 5401 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac 5461 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt 5521 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt 5581 cgcccagctt ctgtatggaa cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa 5641 ggatctggat ttcgatcacg gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg 5701 ggccttgatg ttacccgaga gcttggcacc cagcctgcgc gagcagggga attgatccgg 5761 tggatgacct tttgaatgac ctttaataga ttatattact aattaattgg ggaccctaga 5821 ggtccccttt tttattttaa aaattttttc acaaaacggt ttacaagcat aaagctca//
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