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-20℃
6个月
pDONR-P4P1R
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pDONR-P4P1R 、pDONR P4-P1R |
质粒类型: | Gateway载体;DONR载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | 无 |
克隆方法: | Gateway;BP反应 |
载体大小: | 4777 bp |
5' 测序引物及序列: | M13/pUC fwd 5'CCCAGTCACGACGTTGTAAAACG 3' |
3' 测序引物及序列: | M13/pUC rev 5' AGCGGATAACAATTTCACACAGG 3' |
载体标签: | 无 |
载体抗性: | 卡那霉素,氯霉素(仅空载体) |
真核筛选标记: | 无 |
克隆菌株: | 阳性筛选用DB3.1 ,阴性筛选用OmniMAX2-T1 |
备注: | pDONR-P4P1R载体是MultiSite Gateway载体, 与pDONR221、pDONR-P2RP3载体一同构成三片段系统, 目标载体是pDEST-R4R3,是研究启动子的理想工具。 |
病毒/非病毒: | 非病毒 |
LOCUS Exported 4779 bp ds-DNA circular SYN 10-AUG-2016DEFINITION synthetic circular DNAKEYWORDS pDONR-P4P1R AUTHORS Biofeng Lab.FEATURES Location/Qualifiers source 1..4779 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 268..295 /note="rrnB T2 terminator" /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /note="rrnB T1 terminator" /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 537..553 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" protein_bind 593..824 /gene="mutant version of attP" /bound_moiety="BP Clonase(TM)" /note="attP4" /note="recombination site for the Gateway(R) BP reaction" CDS complement(1181..1486) /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /note="ccdB" /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" CDS complement(1836..2489) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /note="CmR" /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG" promoter complement(2490..2592) /note="cat promoter" /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" protein_bind 2750..2981 /gene="mutant version of attP" /bound_moiety="BP Clonase(TM)" /note="attP1" /note="recombination site for the Gateway(R) BP reaction (pDONR(TM)201 version)" promoter complement(3021..3039) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3044..3060) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 3173..3982 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 4129..4717 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg 601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt 661 ataatgccaa ctttgtatag aaaagttgaa cgagaaacgt aaaatgatat aaatatcaat 721 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatg 781 cagtcactat gaatcaacta cttagatggt attagtgacc tgtagaattc gagctcgacc 841 gacagccttc caaatgttct tctcaaacgg aatcgtcgta tccagcctac tcgctattgt 901 cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa gaggtgcgag cctctttttt 961 gtgtgacaaa ataaaaacat ctacctattc atatacgcta gtgtcatagt cctgaaaatc 1021 atctgcatca agaacaattt cacaactctt atacttttct cttacaagtc gttcggcttc 1081 atctggattt tcagcctcta tacttactaa acgtgataaa gtttctgtaa tttctactgt 1141 atcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca 1201 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga 1261 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga 1321 tatgcaccac cgggtaaagt tcacgggaga ctttatccga cagcagacgt gcactggcca 1381 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca 1441 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg 1501 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc cgtcccttcc 1561 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg 1621 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt 1681 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata 1741 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct 1801 ggcttttagt aagccggatc cacgcggcgt ttacgccccc cctgccactc atcgcagtac 1861 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc 1921 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa 1981 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc 2041 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg 2101 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg 2161 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa 2221 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga 2281 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt 2341 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat 2401 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg 2461 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat 2521 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt 2581 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca 2641 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg 2701 caaagtgcgt cgggtgatgc tgccaactta gtcgacgctc tagagctcca aataatgatt 2761 ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg cttttttata 2821 atgccaactt tgtacaaaaa agttgaacga gaaacgtaaa atgatataaa tatcaatata 2881 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatgcag 2941 tcactatgaa ccaactactt agatggtatt agtgacctgt agaattcgag ctctagagct 3001 gcagggcggc cgcgatatcc cctatagtga gtcgtattac atggtcatag ctgtttcctg 3061 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc 3121 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca 3181 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg 3241 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg 3301 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt 3361 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa 3421 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac 3481 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc 3541 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg 3601 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga 3661 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact 3721 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat 3781 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg 3841 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa 3901 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt 3961 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat 4021 tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc 4081 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 4141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 4201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 4261 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 4321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 4381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 4441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 4501 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga 4561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 4621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 4681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 4741 acggttcctg gccttttgct ggccttttgc tcacatgtt
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