Recombinant Human CD73 (AAH65937.1, C-6His) (C446)
产品说明(Description)
Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.
Accession #: AAH65937.1
Known as: 5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP).
The specific activity is 8000-24000 pmol/min/μg.
Bioactivity-Elisa: |
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Bioactivity-BLI: |
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Immobilized Human CD73-His(Cat#C446) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#NC034). The ED50 of Anti-Human CD73 mAb(Cat#NC034) is 9.92 ng/ml. |
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Loaded Anti-Human CD73 mAb-Fc(Cat#NC034) on Protein A Biosensor, can bind Human CD73-His(Cat#C446) with an affinity constant of <10-3nM as determined in BLI assay. |
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.
电泳(SDS-PAGE)
参考文献(Reference)
An NMR-based Assay for the ex vivo Determination of Soluble CD73 Activity in Serum[J]. Yan He. et al. Analytical Chemistry. 2020.
Selection of LNA-containing DNA aptamers against recombinant human CD73. Elle I C. et al. Molecular BioSystems. 2015.
FOR RESEARCH USE ONLY