Recombinant Rat CD73 (C-6His) (CB16)
产品说明(Description)
Recombinant Rat 5'-Nucleotidase/CD73 is produced by our Mammalian expression system and the target gene encoding Trp29-Lys549 is expressed with a 6His tag at the C-terminus.
Accession #: P21588
Known as: CD73; E5NT;Nucleotidase; NT5E;ecto-5'-nucleotidase
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity-Elisa: |
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Bioactivity-BLI: |
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Immobilized Rat CD73-His(Cat#CB16) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#NC034). The ED50 of Anti-Human CD73 mAb(Cat#NC034) is 8.55 ng/ml. |
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Loaded Anti-Human CD73 mAb-Fc(Cat#NC034) on Protein A Biosensor, can bind Rat CD73-His(Cat#CB16) with an affinity constant of 0.20 nM as determined in BLI assay. |
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
CD73, also known as Ecto-5'-nucleotidase, is an ectoenzyme that is attached to the cell membrane by a glycosyl phophatidylinositol anchor. The enzyme is expressed by most cell types. The 5’-Nucleotidase activity of CD73 converts extracellular nucleoside-5 ’-monophosphates to nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY