Recombinant Mouse Kallikrein 1 (CU31)
产品说明(Description)
Recombinant Mouse Kallikrein-1 is produced by our Mammalian expression system and the target gene encoding Pro19-Asp261 is expressed with a 6His tag at the C-terminus.
Accession #: P15947
Known as: Glandular kallikrein K1; KAL-B; Renal kallikrein; Tissue kallikrein-6; mGK-6
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH7.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Kallikreins belongs to the family of trypsin-like serine proteases, many of which are associated with a variety of cancers. Kallikrein 1 (KLK1) is also known as tissue kallikrein and urinary kallikrein. KLK1 is synthesized as a 261 amino acid (aa) protein that contains a 18 aa signal peptide and a 241 aa proprotein. An important physiological function of KLK1 cleaves Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Kinins regulate vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation.
电泳(SDS-PAGE)
参考文献(References)
Specific phages-based electrochemical impedimetric immunosensors for label-free and ultrasensitive detection of dual prostate-specific antigens[J]. LeiHan. et al. Sensors and Actuators B: Chemical.2019.
FOR RESEARCH USE ONLY