Recombinant Human Isopentenyl pyrophosphate isomerase 2/IDI2 (CE49)
产品说明(Description)
Recombinant Human Isopentenyl Pyrophosphate Isomerase 2 is produced by our E.coli expression system and the target gene encoding Met1-Val227 is expressed with a 6His tag at the N-terminus.
Accession #: Q9BXS1
Known as: Isopentenyl-Diphosphate Delta-Isomerase 2; Isopentenyl Pyrophosphate Isomerase 2; IPP Isomerase 2; IPPI2; IDI2
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 1mM DTT, 0.1mM PMSF, pH 8.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
Isopentenyl Pyrophosphate Isomerase 2 (IDI2) belongs to the IPP isomerase type 1 family. Both isozymes, IDI1 and IDI2 are localized to the peroxisome by a PTS1-dependent pathway. IDI2 is expressed in skeletal muscle, which contains one nudix hydrolase domain. IDI2 binds one magnesium per subunit. IDI2 catalyzes the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP) to its highly electrophilic allylic isomer, dimethylallyl diphosphate . It is reported that IDI2 is regulated independently from IDI1, by a mechanism that may involve PPAR-α.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY