Recombinant Human HGPRT/Hypoxanthine-guanine phosphoribosyltransferase (C294)
产品说明(Description)
Recombinant Human Hypoxanthine-Guanine Phosphoribosyltransferase is produced by our E.coli expression system and the target gene encoding Met1-Ala218 is expressed with a 6His tag at the N-terminus.
Accession #: P00492
Known as: Hypoxanthine-Guanine Phosphoribosyltransferase; HGPRT; HGPRTase; HPRT1; HPRT
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCL, 250mM NaCl, 500mM Imidazole, 30% Glycerol, pH8.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
Hypoxanthine-Guanine Phosphoribosyltransferase (HGPRT) has an important role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 functions to salvage purines from degraded DNA to renewed purine synthesis, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY