Recombinant Human BCAS2/DAM1/Pre-mRNA-splicing factor SPF27 (CE68)
产品说明(Description)
Recombinant Human Breast Carcinoma-Amplified Sequence 2 is produced by our E.coli expression system and the target gene encoding Ala2-Phe225 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
Accession #: O75934
Known as: Pre-mRNA-Splicing Factor SPF27; Breast Carcinoma-Amplified Sequence 2; DNA Amplified in Mammary Carcinoma 1 Protein; Spliceosome-Associated Protein SPF 27; BCAS2; DAM1
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 200mM NaCl, 2mM DTT, pH 8.0.
质量控制(Quality Control)
Purity: Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY