Recombinant Human TGF-beta 1 (CA59)
产品说明(Description)
Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed.
Accession #: P01137
Known as: Transforming Growth Factor Beta-1; TGF-Beta-1; Latency-Associated Peptide; LAP; TGFB1; TGFB; TGF-β1; TGF beta1; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 50mM Glycine-HCl, 150mMNacl, pH2.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.01 EU/ug as determined by LAL test.
Bioactivity:
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Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/ml. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.
电泳(SDS-PAGE)
参考文献(Reference)
RNF2 Mediates Hepatic Stellate Cells Activation by Regulating ERK/p38 Signaling Pathway in LX-2 Cells[J]. Qi Yan. et al. Frontiers in Cell and Developmental Biology. 2021.
METTL3 attenuates proliferative vitreoretinopathy and epithelial-mesenchymal transition of retinal pigment epithelial cells via wnt/β-catenin pathway[J]. Xinqi Ma. et al. Journal of Cellular and Molecular Medicine. 2021.
ERCC6L2 promotes DNA orientation-specific recombination in mammalian cells[J]. Xiaojing Liu. et al. CELL RESEARCH. 2020.
REV7 is required for processing AID initiated DNA lesions in activated B cells[J]. Dingpeng Yang. et al. NATURE COMMUNICATIONS. 2020.
TGF-β1/IL-11/MEK/ERK signaling mediates senescence-associated pulmonary fibrosis in a stress-induced premature senescence model of Bmi-1 deficiency[J]. Haiyun Chen. et al. EXPERIMENTAL AND MOLECULAR MEDICINE. 2020.
Different culture method changing CD105 expression in amniotic fluid MSCs without affecting differentiation ability or immune function[J]. Ding Wang. et al. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2020.
Adipose‐derived stem cells and cancer cells fuse to generate cancer stem cell‐like cells with increased tumorigenicity[J]. Yuk Wah Chan. et al. JOURNAL OF CELLULAR PHYSIOLOGY. 2020.
Enhancement of the antitumor effect of HER2‐directed CAR‐T cells through blocking epithelial‐mesenchymal transition in tumor cells[J]. Peng-Fei Zhang. et al. FFASEB Journal. 2020.
CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis[J]. Chang‐Sheng Zhan. et al. FASEB Journal. 2020.
KrasG12D mutation contributes to regulatory T cell conversion through activation of the MEK/ERK pathway in pancreatic cancer[J]. He Cheng. et al. Cancer Letters. 2019.
FOR RESEARCH USE ONLY