Recombinant Human IGF2 (CF61)
产品说明(Description)
Recombinant Human Long Insulin-Like Growth Factor II is produced by our E.coli expression system and the target gene encoding Ala25-Glu91 is expressed.
Accession #: P01344
Known as: Insulin-Like Growth Factor II; IGF-II; Somatomedin-A; IGF2; PP1446
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 5mM Hac, pH 3.0.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells.The ED50 for this effect is 15.7 ng/ml.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Insulin-Like Growth Factor II (IGF2) belongs to the insulin family of polypeptide growth factors that is involved in development and growth. Members of this family are structurally homologous to proinsulin, and share higher sequence identity. IGF2 is expressed only from the paternally inherited allele and believed to be secreted by the liver and to circulate in the blood. IGF2 possess growth-promoting activity and can stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF2 is influenced by placental lactogen and may play a role in fetal development.
电泳(SDS-PAGE)
参考文献(References)
Regulation of chitosan-mediated differentiation of human olfactory receptor neurons by insulin-like growth factor binding protein-2[J]. Tsung-Wei Huang. et al. Acta Biomaterialia. 2019.
RNA‐Binding Protein IGF2BP2/IMP2 is a Critical Maternal Activator in Early Zygotic Genome Activation[J]. Hong-Bin Liu. et al. Advanced Science. 2019.
FOR RESEARCH USE ONLY