目录号:DM005-01A
产品描述:
DNA Ladder 2000由6条线状双链DNA片段组成,其中750bp条带浓度约为30ng/μl,显示为亮带,使电泳条带更容易辨认,亦可用于定量。其他各条带浓度约为10ng/μl。本制品已混有1×Loading Buffer,可直接用于凝胶电泳。
浓度:80ng/μl
条带组成:100bp,250bp,500bp,750bp(加亮),1000bp,2000bp。
使用建议:
建议取5μl加入加样孔中(或按每毫米胶孔宽度上1μl量)。
建议用1.5%Agrose,电压4-10V/cm,1×TAE或0.5×TBE进行琼脂糖凝胶电泳。
可以通过EB或者其他核酸染料进行染色,在紫外灯下观察电泳条带。
保存温度:
4℃保存3个月,-20℃保存2年。
注意事项:
1)使用高品质的琼脂糖,并及时更换电泳缓冲液,以免影响电泳效果。
2)使用本制品进行定量分析时,需将样品进行梯度上样,以保证结果准确。
3) 使用含有EB染料的琼脂糖凝胶进行电泳检测时,待溴酚蓝染料到约2/3处即可停止电泳,以防小条带脱离EB造成条带变暗。
参考文献:
Improved RAPD Analysis of Canarium album (Lour.) Raeusch from Sichuan Province along Yangtze River in China[J]. Mei Z et al. Annual Review & Research in Biology. 2014.
Intracellular generation of single-strand template increases the knock-in efficiency by combining CRISPR/Cas9 with AAV[J]. Qing Xiao et al. Molecular Genetics and Genomics. 2018.
Non-chromatographic purification of thermostable endoglucanase from Thermotoga maritima by fusion with a hydrophobic elastin-like polypeptide[J]. Shanshan Wang. et al. Protein Expression and Purification. 2020.
Genetic characterization and authentication of Lonicera japonica Thunb. by using improved RAPD analysis[J]. Fu J et al. Molecular biology reports. 2013.
Genetic characterization and authentication of Gardenia jasminoides in different regions of China by using improved RAPD analysis[J]. Mei Z et al. Indian journal of experimental biology. 2015.
For research use only.