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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK0373 |
---|---|
产品名称 | Human IFN Gamma/IFNG ELISA KIt |
产品描述 | For quantitative detection of human IFN gamma in cell culture supernates and serum. |
免疫原 | E.coli, Q24-Q166 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | IFNG |
基因全名 | interferon, gamma |
Uniprot ID | IFNG: P01579 |
指标别名 | IFG|IFI|IFN gamma|IFN Immune|IFNG|Interferon gamma|Interferon Gamma Precursor|Macrophage Activating Factor|MAF|T Cell Interferon|Type II Interferon |
背景资料 | Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0373-CAP | 96-well plate precoated with anti- human IFN gamma antibody | 1 |
EK0373-ST | Lyophilized recombinant human IFN gamma standard | 10ng/tube×2 |
EK0373-DA | Biotinylated anti- human IFN gamma antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 34 | 150 | 469 | 33 | 139 | 437 |
Standard deviation | 1.73 | 8.7 | 29.54 | 1.91 | 9.73 | 31.9 |
CV(%) | 5.1% | 5.8% | 6.3% | 5.8% | 7% | 7.3% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.011 | 0.056 | 0.098 | 0.183 | 0.366 | 0.646 | 1.382 | 2.405 |
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IFN R.pdf 附 (下载 17 次)