Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGDF-3. Anti-Human GDF-3 specific antibody was purified by affinity chromatography and then biotinylated.
Catalog #:
500-P235Bt
Source :
Rabbits
Formulation :
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Stability :
The lyophilized antibody is stable for at least 2 years from date of receipt at -200 C. The reconstituted antibody is stable for at least two weeks at 2-80 C. Frozen aliquots are stable for at least 6 months when stored at -200 C. Avoid repeated freeze-thaw cycles.
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Applications :
Elisa
Direct:To detect hGDF-3 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGDF-3. Sandwich:To detect hGDF-3 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ’s Polyclonal Anti-Human GDF-3 (500-P235) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hGDF-3.
Western Blot
To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGDF-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Country of Origin :
USA
Size A
25µg
$195
Size B
50µg
$250
Size C
1mg
$4000
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Usage: For Research Use Only. Not for use in diagnostic or therapeutic procedures.