Produced in BALB/c mice using highly pure (>98%) recombinant rat IL-2 as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Catalog #:
500-M129
Source :
Mice
Formulation :
A sterile filtered antibody solution was lyophilized from PBS.
Stability :
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20o C. Frozen aliquots are stable for at least six weeks at 2-8o C or for at least six months at -20o C. Avoid repeated freeze-thaw cycles.
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml.
Applications :
Neutralization
Data Not Available.
Elisa
In a sandwich ELISA (assuming 100µl/well), a concentration of 14.0-16.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant rat IL-2 when used with 's biotinylated antigen affinity purified anti-rat IL-2 (500-P274Bt) as the detection antibody at a concentration of at least 2.0-4.0 µg/ml.
Western Blot
To detect rat IL-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-2 is 2.0-4.0 ng/lane, under reducing or non-reducing conditions.
Country of Origin :
USA
Size B
500µg
$150
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Usage: For Research Use Only. Not for use in diagnostic or therapeutic procedures.