Organism SpeciesHomo sapiens (Human) Same name, Different species.
Sample Typen/a
Test MethodDouble-antibody Sandwich
Assay Length1h, 10min
Detection Rangen/a
Sensitivityn/a
SpecificityThis assay has high sensitivity and excellent specificity for detection of Instant Interleukin 8 (IL8). No significant cross-reactivity or interference between Instant Interleukin 8 (IL8) and analogues was observed.
ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
1. Prepare all reagents, samples and standards; 2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C; 3. Aspirate and wash 3 times; 4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 7. Add 50µL Stop Solution. Read at 450nm immediately.