pOnebyOne™ I – Lentiviral Bicistronic Mammalian Expression Kit
Extremely Efficient lineal vector ready-to-clone your PCR gene and express your Protein of Interest
Product Overview:
pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ I – Lentiviral includes the Human cytomegalovirus promoter that precedes 2A sequence in frame with the truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones as they can be visualized by cytometry using a specific antibody labelled with FITC or using a similar method, and also, they can be enriched from negative clones with magnetic beads bearing an anti ΔNGFR antibody.
Advantages & Features
Complete solution: a directional cloning vector to clone and produce a protein of interest.
Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter).
Highly efficient: cloning system tested with up to 4 kb inserts.
Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment.
Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest.
Cost avoidance: avoids the use of restriction enzymes.
Accurate: proven performance for most common cell lines.
Flexible: allows transfection in difficult cell lines.
Really low experimental background: lower than 1%.
Convenient: available with different resistance marker cassettes.
Directional cloning: of PCR with the gene of interest.
Reporter checking: stoichiometric amount of your protein of interest and a reporter protein.