Small RNA Analysis - Details & Specifications
The Small RNA kit for the Agilent 2100 Bioanalyzer provides a fast and sensitive analysis to resolve small nucleic acids, typical applications are:
- Analyze miRNA directly in total RNA or in enriched samples
- Compare small RNA regions in extracts and tissues
- Separate miRNA from other small RNAs.
- Evaluate miRNA yields while optimizing extraction parameters
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The electropherogram shows the analysis of the small RNA content (75 ng/µL) in a thymus total RNA sample. The miRNA and the small RNA region are indicated. (Application Note 5989-7870EN) |
Analytical specifications |
Small RNA kit |
total RNA |
Quantitative range |
50-2000 pg/µL of purified miRNA in water |
Qualitative range |
50-2000 pg/µL of purified miRNA in water |
Size range |
6-150 nt |
Sensitivity (S/N>3)1 |
50 pg/µL in water |
Quantitation reproducibility (within a chip) |
25% CV |
Quantitation accuracy |
- |
Maximum sample buffer strength |
10 mM Tris 0.1 mM EDTA |
Physical specifications |
Analysis time |
30 minutes |
Samples per chip |
11 |
Sample volume |
1 µL |
Kit stability |
≥4 months at 4°C |
Kit size |
25 chips 11 samples/chip = 275 samples/kit |