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- 英文名:
High Sensitivity DNA Analysis Kits
- 库存:
现货
- 供应商:
广州柏赛柯生物
High Sensitivity DNA Analysis Kits
客服热线:020-39341005
联系客服QQ: 1195082744

The High Sensitivity DNA kit allows analysis of fragmented DNA or DNA libraries.
- Increased sensitivity - down to 5 pg/µL
- Analysis of complex DNA samples - down to 100 pg/µL for fragmented DNA or DNA libraries
- Broad linear dynamic range - detection of less abundant PCR products
- Smear analysis - specify size range of interest
- Quantitation accuracy and reproducibility - quantitation of each DNA fragment against internal standards
- Minimal sample consumption - only 1 µL of sample required
High Sensitivity DNA Analysis Kits - Details & Specifications
With the High Sensitivity DNA kit, DNA libraries derived from just a few amplification cycles can easily be analyzed, reducing amplification bias and improving the quality of sequencing data (see electropherogram overlay). In addition, the kit offers improved sensitivity for checking the size and quantity of precious DNA samples of limited abundance. Whether fragmented DNA or DNA sequencing libraries, the High Sensitivity DNA kit reliably sizes and quantifies in the lower pg/µL concentration range.
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Overlay of DNA electropherograms obtained after 4 to 10 PCR cycles. The amplified DNA could be detected and reliably quantified, even after only four PCR cycles. (Application Note 5990-5008EN) |
| Analytical specifications | High Sensitivity DNA Kit |
| Sizing range | 50 – 7000 bp |
| Sizing resolution | 50-600 bp: ±10% 600-7000 bp:± 20% |
| Sizing accuracy | ± 10% |
| Sizing reproducibility | 5% CV |
| Quantitation accuracy1 | 20% |
| Quantitation reproducibility1 | 50-2000 bp: 15% CV 2000-7000 bp: 10% CV |
| Quantitative range1 | 5-500 pg/µL |
| Maximum salt concentration in sample | 10 mM Tris and 1 mM EDTA |
| Physical specifications | |
| Analysis time | 45 minutes |
| Samples per chip | 11 |
| Sample volume | 1 µL |
| Kit stability | 4 months (see box for storage temperatures) |
| Kit size | 10 chips 11 samples/chip = 110 samples/kit |
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