Background: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform. Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Belongs to the PI3/PI4-kinase family. Located to cytosol, lamellipodium and phosphatidylinositol 3-kinase complex.
Immunogen: A synthetic peptide from the internal region of PIK3CA (Q546K) of human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: recognizes Q546K mutant, but not wildtype PIK3CA of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of PIK3CA wild type and Q546K proteins. Equal amount of purified PIK3CA (Q546K)(amino acids 461-640) and corresponding wild type proteins were loaded and blotted with anti- PIK3CA (Q546K) mouse monoclonal antibody(Cat. # 26138).