Background: Anaplastic lymphoma kinase (ALK) also known as CD246 (cluster of differentiation 246) is a tyrosine kinase receptor that plays an important role in the development of the brain and nervous systems. Deficiencies in ALK, which most commonly as a fusion with other genes, are indicated in cancers.
Immunogen: A synthetic peptide from the internal region of ALK which includes the mutation of C1156Y, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes C1156Y mutant, but not wild-type ALK of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant ALK (C1156Y) and wildtype proteins. Purified His-tagged ALK (C1156Y) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-ALK (C1156Y) monoclonal antibody (Cat. #26226).
Immunofluorescence:
Immunofluorescence of cells expressing ALK proteins with anti-ALK (C1156Y) antibody. HEK293T cells were transfected with pCDNA3-GFP-ALK (WT) plasmid (left column) or pCDNA3-GFP-ALK (C1156Y) plasmid (right column), then fixed and stained with anti-ALK (C1156Y) monoclonal antibody (Cat. #26226).