Background: Ras (G12D) is one type of Ras missense mutations at the codon 12. Ras protein is a membrane-associated GTPase that regulates cell proliferation, differentiation, and survival. Ras G12D mutation results in decreased GTPase activity and constitutive signaling. This mutation is found in many types of human cancers.
Immunogen: A synthetic peptide from the internal region of Ras which includes the mutation of G12D, human origin.
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Ras(G12D) antibody recognizes Ras(G12D) proteins of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant Ras WT and G12D proteins. Equal amounts of purified Ras WT (lane 1, Cat # 10172) and G12D (lane 2, Cat # 10154) proteins were loaded and blotted with anti-Ras G12D mouse monoclonal antibody (Cat # 26036) (Top panel). Input control (bottom panel) was blotted with rabbit polyclonal anti-Ras antibody (Cat # 21021).
Immunofluorescence:
Immunofluorescence of cells expressing RAS proteins with anti-RAS (G12D) antibody. HEK293T cells were transfected with pCDNA3-GFP-Ras(G12D) plasmid, pCDNA3-GFP-Ras(WT)plasmid,pCDNA3-GFP-Ras(G12S) plasmid, pCDNA3-GFP-Ras(G12V)plasmid,pCDNA3-GFP-Ras(G12C)plasmid, pCDNA3-GFP-Ras(G12R)plasmid,or pCDNA3-GFP-Ras(G12A)plasmid, then fixed and stained with anti-Ras(G12D) monoclonal antibody (Cat. # 26036).