Background: Arl3 (Arf-like 3) is an Arf family protein. Arl3 binds microtubules to alter specific aspects of cytokinesis. It has been proposed that retinitis pigmentosa 2 functions in concert with Arl3 to link the cell membrane and the cytoskeleton in photoreceptors as part of the cell signaling or vesicular transport machinery.
Immunogen: Recombinant Arl3 protein (active form)
Applications: IP, IHC, IF
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC, IF
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Arl3 antibody recognizes active Arl3-GTP from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Arl3 Activation Assay. Purified His-tagged Arl3 proteins (Cat. #10152) were immunoprecipitated with the anti-active Arl3 monoclonal antibody (Cat. #26925) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti-Arl3 monoclonal antibody (Cat. #21070). Input controls are shown in the bottom panel.