Background: ARF6 (ADP-ribosylation factor 6) is a member of the ARF super-family. ARF genes encode small GTPases that increase the ADP-ribosyltransferase activity of cholera toxin and are critical for vesicular trafficking as activators of phospholipase D. Arf6 regulates membrane trafficking and functions as a regulatory molecule of phagocytosis.
Immunogen: Recombinant full length protein of active Arf6.
Applications: IP, IHC
Recommended Dilutions: IP: 1 μg for 1~2 mg total cellular proteins IHC: 1:50~100
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Arf6 antibody recognizes active Arf6 from vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Purified full-length Arf6 proteins were immunoprecipitated after treated with GDP (lane left) or GTPγS (lane right). Immunoprecipitation was done with the anti-active Arf6 monoclonal antibody (Cat. No. 26918). Immunoblot was with an anti-Arf6 polyclonal antibody (Cat. No. 21032).