Description: Anti-Active Ran Mouse Monoclonal Antibody
Background: Ran (Ras-related nuclear protein) is a member of the Ras-superfamily GTPases. Ran is invovled in the transport of proteins across the nuclear envelope, as well as in microtubule organization during mitosis.
Immunogen: Recombinant full length protein of active Ran.
Applications: IP, IHC
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC
Concentration: 1 mg/ml
Host: mouse
Format: Liquid
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti-active Ran antibody recognizes active Ran of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Immunoprecipitation/Western blot:
Ran activation assay. Purified Ran proteins were loaded as a control (lanes 1) or immunoprecipitated after treated with GDP (lane 2) or GTPγS (lane 3). Immunoprecipitation was done with the anti-active Ran monoclonal antibody (Cat. No. 26915). Immunoblot was with an anti-Ran polyclonal antibody.