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pET-28b(+)
大量
信裕生物
名称 | 编号 | 规格 | 价格 | 手册 |
---|---|---|---|---|
pET-28b(+)(pET28b)(60908-2819)y | 60908-2819y |
质粒编号: 60908-2819y
质粒名称: pET-28b(+)
其他名称:
质粒大小(bp): 5368
抗性: Kan
质粒类型: Bacterial Expression
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: His (Nterm and Cterm)
测序引物:
GenBank号:
其他属性: N term thrombin cleavage site; a,b,c vary by MCS;
质粒图谱:
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
T7_leader | 238 | 206 | |
Xpress_fwd_primer | 239 | 221 | |
T7_transl_en_RBS | 322 | 306 | |
lacO | 367 | 340 | |
T7_promoter | 385 | 367 | |
tet (300 – 563) | 417 | 680 | |
pBRrevBam_primer | 488 | 469 | |
lacI | 763 | 1854 | |
tet (576 – 851) | 1913 | 2188 | |
ROP | 2663 | 2854 | |
pGEX_3_primer | 2870 | 2848 | |
pBR322_origin | 3888 | 3269 | |
KanR2 | 3994 | 4809 |
ORF | Start | End | |
---|---|---|---|
ORF frame 3 | 522 | 1022 | |
ORF frame 1 | 895 | 1854 | |
ORF frame 1 | 3994 | 4809 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NheI | 230 |
NdeI | 237 |
NcoI | 295 |
XbaI | 334 |
BglII | 400 |
BclI | 1136 |
ApaI | 1333 |
EcoRV | 1572 |
HpaI | 1628 |
FspI | 2204 |
NruI | 4082 |
ClaI | 4116 |
XmaI | 4297 |
SmaI | 4299 |
质粒序列:
1 | ATCCGGATAT | AGTTCCTCCT | TTCAGCAAAA | AACCCCTCAA | GACCCGTTTA | 50 |
51 | GAGGCCCCAA | GGGGTTATGC | TAGTTATTGC | TCAGCGGTGG | CAGCAGCCAA | 100 |
101 | CTCAGCTTCC | TTTCGGGCTT | TGTTAGCAGC | CGGATCTCAG | TGGTGGTGGT | 150 |
151 | GGTGGTGCTC | GAGTGCGGCC | GCAAGCTTGT | CGACGGAGCT | CGAATTCGGA | 200 |
201 | TCCCGACCCA | TTTGCTGTCC | ACCAGTCATG | CTAGCCATAT | GGCTGCCGCG | 250 |
251 | CGGCACCAGG | CCGCTGCTGT | GATGATGATG | ATGATGGCTG | CTGCCCATGG | 300 |
301 | TATATCTCCT | TCTTAAAGTT | AAACAAAATT | ATTTCTAGAG | GGGAATTGTT | 350 |
351 | ATCCGCTCAC | AATTCCCCTA | TAGTGAGTCG | TATTAATTTC | GCGGGATCGA | 400 |
401 | GATCTCGATC | CTCTACGCCG | GACGCATCGT | GGCCGGCATC | ACCGGCGCCA | 450 |
451 | CAGGTGCGGT | TGCTGGCGCC | TATATCGCCG | ACATCACCGA | TGGGGAAGAT | 500 |
501 | CGGGCTCGCC | ACTTCGGGCT | CATGAGCGCT | TGTTTCGGCG | TGGGTATGGT | 550 |
551 | GGCAGGCCCC | GTGGCCGGGG | GACTGTTGGG | CGCCATCTCC | TTGCATGCAC | 600 |
601 | CATTCCTTGC | GGCGGCGGTG | CTCAACGGCC | TCAACCTACT | ACTGGGCTGC | 650 |
651 | TTCCTAATGC | AGGAGTCGCA | TAAGGGAGAG | CGTCGAGATC | CCGGACACCA | 700 |
701 | TCGAATGGCG | CAAAACCTTT | CGCGGTATGG | CATGATAGCG | CCCGGAAGAG | 750 |
751 | AGTCAATTCA | GGGTGGTGAA | TGTGAAACCA | GTAACGTTAT | ACGATGTCGC | 800 |
801 | AGAGTATGCC | GGTGTCTCTT | ATCAGACCGT | TTCCCGCGTG | GTGAACCAGG | 850 |
851 | CCAGCCACGT | TTCTGCGAAA | ACGCGGGAAA | AAGTGGAAGC | GGCGATGGCG | 900 |
901 | GAGCTGAATT | ACATTCCCAA | CCGCGTGGCA | CAACAACTGG | CGGGCAAACA | 950 |
951 | GTCGTTGCTG | ATTGGCGTTG | CCACCTCCAG | TCTGGCCCTG | CACGCGCCGT | 1000 |
1001 | CGCAAATTGT | CGCGGCGATT | AAATCTCGCG | CCGATCAACT | GGGTGCCAGC | 1050 |
1051 | GTGGTGGTGT | CGATGGTAGA | ACGAAGCGGC | GTCGAAGCCT | GTAAAGCGGC | 1100 |
1101 | GGTGCACAAT | CTTCTCGCGC | AACGCGTCAG | TGGGCTGATC | ATTAACTATC | 1150 |
1151 | CGCTGGATGA | CCAGGATGCC | ATTGCTGTGG | AAGCTGCCTG | CACTAATGTT | 1200 |
1201 | CCGGCGTTAT | TTCTTGATGT | CTCTGACCAG | ACACCCATCA | ACAGTATTAT | 1250 |
1251 | TTTCTCCCAT | GAAGACGGTA | CGCGACTGGG | CGTGGAGCAT | CTGGTCGCAT | 1300 |
1301 | TGGGTCACCA | GCAAATCGCG | CTGTTAGCGG | GCCCATTAAG | TTCTGTCTCG | 1350 |
1351 | GCGCGTCTGC | GTCTGGCTGG | CTGGCATAAA | TATCTCACTC | GCAATCAAAT | 1400 |
1401 | TCAGCCGATA | GCGGAACGGG | AAGGCGACTG | GAGTGCCATG | TCCGGTTTTC | 1450 |
1451 | AACAAACCAT | GCAAATGCTG | AATGAGGGCA | TCGTTCCCAC | TGCGATGCTG | 1500 |
1501 | GTTGCCAACG | ATCAGATGGC | GCTGGGCGCA | ATGCGCGCCA | TTACCGAGTC | 1550 |
1551 | CGGGCTGCGC | GTTGGTGCGG | ATATCTCGGT | AGTGGGATAC | GACGATACCG | 1600 |
1601 | AAGACAGCTC | ATGTTATATC | CCGCCGTTAA | CCACCATCAA | ACAGGATTTT | 1650 |
1651 | CGCCTGCTGG | GGCAAACCAG | CGTGGACCGC | TTGCTGCAAC | TCTCTCAGGG | 1700 |
1701 | CCAGGCGGTG | AAGGGCAATC | AGCTGTTGCC | CGTCTCACTG | GTGAAAAGAA | 1750 |
1751 | AAACCACCCT | GGCGCCCAAT | ACGCAAACCG | CCTCTCCCCG | CGCGTTGGCC | 1800 |
1801 | GATTCATTAA | TGCAGCTGGC | ACGACAGGTT | TCCCGACTGG | AAAGCGGGCA | 1850 |
1851 | GTGAGCGCAA | CGCAATTAAT | GTAAGTTAGC | TCACTCATTA | GGCACCGGGA | 1900 |
1901 | TCTCGACCGA | TGCCCTTGAG | AGCCTTCAAC | CCAGTCAGCT | CCTTCCGGTG | 1950 |
1951 | GGCGCGGGGC | ATGACTATCG | TCGCCGCACT | TATGACTGTC | TTCTTTATCA | 2000 |
2001 | TGCAACTCGT | AGGACAGGTG | CCGGCAGCGC | TCTGGGTCAT | TTTCGGCGAG | 2050 |
2051 | GACCGCTTTC | GCTGGAGCGC | GACGATGATC | GGCCTGTCGC | TTGCGGTATT | 2100 |
2101 | CGGAATCTTG | CACGCCCTCG | CTCAAGCCTT | CGTCACTGGT | CCCGCCACCA | 2150 |
2151 | AACGTTTCGG | CGAGAAGCAG | GCCATTATCG | CCGGCATGGC | GGCCCCACGG | 2200 |
2201 | GTGCGCATGA | TCGTGCTCCT | GTCGTTGAGG | ACCCGGCTAG | GCTGGCGGGG | 2250 |
2251 | TTGCCTTACT | GGTTAGCAGA | ATGAATCACC | GATACGCGAG | CGAACGTGAA | 2300 |
2301 | GCGACTGCTG | CTGCAAAACG | TCTGCGACCT | GAGCAACAAC | ATGAATGGTC | 2350 |
2351 | TTCGGTTTCC | GTGTTTCGTA | AAGTCTGGAA | ACGCGGAAGT | CAGCGCCCTG | 2400 |
2401 | CACCATTATG | TTCCGGATCT | GCATCGCAGG | ATGCTGCTGG | CTACCCTGTG | 2450 |
2451 | GAACACCTAC | ATCTGTATTA | ACGAAGCGCT | GGCATTGACC | CTGAGTGATT | 2500 |
2501 | TTTCTCTGGT | CCCGCCGCAT | CCATACCGCC | AGTTGTTTAC | CCTCACAACG | 2550 |
2551 | TTCCAGTAAC | CGGGCATGTT | CATCATCAGT | AACCCGTATC | GTGAGCATCC | 2600 |
2601 | TCTCTCGTTT | CATCGGTATC | ATTACCCCCA | TGAACAGAAA | TCCCCCTTAC | 2650 |
2651 | ACGGAGGCAT | CAGTGACCAA | ACAGGAAAAA | ACCGCCCTTA | ACATGGCCCG | 2700 |
2701 | CTTTATCAGA | AGCCAGACAT | TAACGCTTCT | GGAGAAACTC | AACGAGCTGG | 2750 |
2751 | ACGCGGATGA | ACAGGCAGAC | ATCTGTGAAT | CGCTTCACGA | CCACGCTGAT | 2800 |
2801 | GAGCTTTACC | GCAGCTGCCT | CGCGCGTTTC | GGTGATGACG | GTGAAAACCT | 2850 |
2851 | CTGACACATG | CAGCTCCCGG | AGACGGTCAC | AGCTTGTCTG | TAAGCGGATG | 2900 |
2901 | CCGGGAGCAG | ACAAGCCCGT | CAGGGCGCGT | CAGCGGGTGT | TGGCGGGTGT | 2950 |
2951 | CGGGGCGCAG | CCATGACCCA | GTCACGTAGC | GATAGCGGAG | TGTATACTGG | 3000 |
3001 | CTTAACTATG | CGGCATCAGA | GCAGATTGTA | CTGAGAGTGC | ACCATATATG | 3050 |
3051 | CGGTGTGAAA | TACCGCACAG | ATGCGTAAGG | AGAAAATACC | GCATCAGGCG | 3100 |
3101 | CTCTTCCGCT | TCCTCGCTCA | CTGACTCGCT | GCGCTCGGTC | GTTCGGCTGC | 3150 |
3151 | GGCGAGCGGT | ATCAGCTCAC | TCAAAGGCGG | TAATACGGTT | ATCCACAGAA | 3200 |
3201 | TCAGGGGATA | ACGCAGGAAA | GAACATGTGA | GCAAAAGGCC | AGCAAAAGGC | 3250 |
3251 | CAGGAACCGT | AAAAAGGCCG | CGTTGCTGGC | GTTTTTCCAT | AGGCTCCGCC | 3300 |
3301 | CCCCTGACGA | GCATCACAAA | AATCGACGCT | CAAGTCAGAG | GTGGCGAAAC | 3350 |
3351 | CCGACAGGAC | TATAAAGATA | CCAGGCGTTT | CCCCCTGGAA | GCTCCCTCGT | 3400 |
3401 | GCGCTCTCCT | GTTCCGACCC | TGCCGCTTAC | CGGATACCTG | TCCGCCTTTC | 3450 |
3451 | TCCCTTCGGG | AAGCGTGGCG | CTTTCTCATA | GCTCACGCTG | TAGGTATCTC | 3500 |
3501 | AGTTCGGTGT | AGGTCGTTCG | CTCCAAGCTG | GGCTGTGTGC | ACGAACCCCC | 3550 |
3551 | CGTTCAGCCC | GACCGCTGCG | CCTTATCCGG | TAACTATCGT | CTTGAGTCCA | 3600 |
3601 | ACCCGGTAAG | ACACGACTTA | TCGCCACTGG | CAGCAGCCAC | TGGTAACAGG | 3650 |
3651 | ATTAGCAGAG | CGAGGTATGT | AGGCGGTGCT | ACAGAGTTCT | TGAAGTGGTG | 3700 |
3701 | GCCTAACTAC | GGCTACACTA | GAAGGACAGT | ATTTGGTATC | TGCGCTCTGC | 3750 |
3751 | TGAAGCCAGT | TACCTTCGGA | AAAAGAGTTG | GTAGCTCTTG | ATCCGGCAAA | 3800 |
3801 | CAAACCACCG | CTGGTAGCGG | TGGTTTTTTT | GTTTGCAAGC | AGCAGATTAC | 3850 |
3851 | GCGCAGAAAA | AAAGGATCTC | AAGAAGATCC | TTTGATCTTT | TCTACGGGGT | 3900 |
3901 | CTGACGCTCA | GTGGAACGAA | AACTCACGTT | AAGGGATTTT | GGTCATGAAC | 3950 |
3951 | AATAAAACTG | TCTGCTTACA | TAAACAGTAA | TACAAGGGGT | GTTATGAGCC | 4000 |
4001 | ATATTCAACG | GGAAACGTCT | TGCTCTAGGC | CGCGATTAAA | TTCCAACATG | 4050 |
4051 | GATGCTGATT | TATATGGGTA | TAAATGGGCT | CGCGATAATG | TCGGGCAATC | 4100 |
4101 | AGGTGCGACA | ATCTATCGAT | TGTATGGGAA | GCCCGATGCG | CCAGAGTTGT | 4150 |
4151 | TTCTGAAACA | TGGCAAAGGT | AGCGTTGCCA | ATGATGTTAC | AGATGAGATG | 4200 |
4201 | GTCAGACTAA | ACTGGCTGAC | GGAATTTATG | CCTCTTCCGA | CCATCAAGCA | 4250 |
4251 | TTTTATCCGT | ACTCCTGATG | ATGCATGGTT | ACTCACCACT | GCGATCCCCG | 4300 |
4301 | GGAAAACAGC | ATTCCAGGTA | TTAGAAGAAT | ATCCTGATTC | AGGTGAAAAT | 4350 |
4351 | ATTGTTGATG | CGCTGGCAGT | GTTCCTGCGC | CGGTTGCATT | CGATTCCTGT | 4400 |
4401 | TTGTAATTGT | CCTTTTAACA | GCGATCGCGT | ATTTCGTCTC | GCTCAGGCGC | 4450 |
4451 | AATCACGAAT | GAATAACGGT | TTGGTTGATG | CGAGTGATTT | TGATGACGAG | 4500 |
4501 | CGTAATGGCT | GGCCTGTTGA | ACAAGTCTGG | AAAGAAATGC | ATAAACTTTT | 4550 |
4551 | GCCATTCTCA | CCGGATTCAG | TCGTCACTCA | TGGTGATTTC | TCACTTGATA | 4600 |
4601 | ACCTTATTTT | TGACGAGGGG | AAATTAATAG | GTTGTATTGA | TGTTGGACGA | 4650 |
4651 | GTCGGAATCG | CAGACCGATA | CCAGGATCTT | GCCATCCTAT | GGAACTGCCT | 4700 |
4701 | CGGTGAGTTT | TCTCCTTCAT | TACAGAAACG | GCTTTTTCAA | AAATATGGTA | 4750 |
4751 | TTGATAATCC | TGATATGAAT | AAATTGCAGT | TTCATTTGAT | GCTCGATGAG | 4800 |
4801 | TTTTTCTAAG | AATTAATTCA | TGAGCGGATA | CATATTTGAA | TGTATTTAGA | 4850 |
4851 | AAAATAAACA | AATAGGGGTT | CCGCGCACAT | TTCCCCGAAA | AGTGCCACCT | 4900 |
4901 | GAAATTGTAA | ACGTTAATAT | TTTGTTAAAA | TTCGCGTTAA | ATTTTTGTTA | 4950 |
4951 | AATCAGCTCA | TTTTTTAACC | AATAGGCCGA | AATCGGCAAA | ATCCCTTATA | 5000 |
5001 | AATCAAAAGA | ATAGACCGAG | ATAGGGTTGA | GTGTTGTTCC | AGTTTGGAAC | 5050 |
5051 | AAGAGTCCAC | TATTAAAGAA | CGTGGACTCC | AACGTCAAAG | GGCGAAAAAC | 5100 |
5101 | CGTCTATCAG | GGCGATGGCC | CACTACGTGA | ACCATCACCC | TAATCAAGTT | 5150 |
5151 | TTTTGGGGTC | GAGGTGCCGT | AAAGCACTAA | ATCGGAACCC | TAAAGGGAGC | 5200 |
5201 | CCCCGATTTA | GAGCTTGACG | GGGAAAGCCG | GCGAACGTGG | CGAGAAAGGA | 5250 |
5251 | AGGGAAGAAA | GCGAAAGGAG | CGGGCGCTAG | GGCGCTGGCA | AGTGTAGCGG | 5300 |
5301 | TCACGCTGCG | CGTAACCACC | ACACCCGCCG | CGCTTAATGC | GCCGCTACAG | 5350 |
5351 | GGCGCGTCCC | ATTCGCCA | 5368 |
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