手机验证
0
0
0
0
单克隆
human
0
3-4周
博士德生物
WB,IF,ELISA,Dot blot,ChIP-seq,ChIP-qPCR,ChIP
0
50μg/42μl
产品名称 | H3K27ac Antibody ChIP Grade |
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产品描述 | Rabbit IgG polyclonal antibody for histone H3, acetylated at lysine 27 (H3K27ac). Tested with ChIP,ChIP-seq,ELISA,Dot blot,WB,IF in Human. |
文献引用格式 | H3K27ac Antibody ChIP Grade (Boster Biological Technology, Wuhan, China. Catalog #CI1073) |
应用范围 | WB, IF, ELISA, Dot blot, ChIP-seq, ChIP-qPCR, ChIP |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation. *Blocking peptide 可以联系我们购买。¥300/1ml |
免疫原 | This antibody is raised in rabbit against histone H3, acetylated at lysine 27 (H3K27ac), using a KLH-conjugated synthetic peptide. |
抗体规格 | 50μg/42μl |
克隆 | Polyclonal |
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产品形态 | Liquid |
内容 | Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300. *可以提供无载体抗体。 |
储存条件 | At -20℃ for one year, at 4℃ for one month. Avoid repeated freezing and thawing. |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | HIST1H3A |
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基因全名 | Histone H3.1 |
蛋白功能 | Core component of nucleosome. Nucleosomes wrap andcompact DNA into chromatin, limiting DNA accessibility to thecellular machineries which require DNA as a template. Histonesthereby play a central role in transcription regulation, DNArepair, DNA replication and chromosomal stability. DNAaccessibility is regulated via a complex set of post-translationalmodifications of histones, also called histone code, andnucleosome remodeling. |
Tissue Specificity | |
Sequence Similarities | |
Uniprot ID | HIST1H3A: P68431 |
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called €histone code€. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
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[list_product_images]ChIP assays were performed using HeLa cells, Anti-H3K27ac polyclonal antibody (Catalog # CI1073) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 2, 5 and 10μg per ChIP experiment was analysed. IgG (2μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for the promoter of the active ACTB and EIF4A2 genes, used as positive controls, and for the coding region of the inactive MYT1 and TSH2B genes, used as negative controls.|A Dot Blot analysis was performed to test the cross reactivity of Anti-H3K27ac polyclonal antibody (Catalog # CI1073) with peptides containing other histone modifications and the unmodified H3K27 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:25,000. This figure shows a high specificity of the antibody for the modification of interest.|To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-H3K27ac polyclonal antibody (Catalog # CI1073) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:4,000.|Western blot analysis of H3K27ac expression in HeLa whole cell lysates (40 μg, lane 1), histone extracts from HeLa cells (15 μg, lane 2), recombinant histone H2A (1 μg, lane 3), recombinant histone H2B (1 μg, lane 4), recombinant histone H3 (1 μg, lane 5) and recombinant histone H4 (1 μg, lane 6). H3K27ac at 17KD was detected using Anti-H3K27ac polyclonal antibody (Catalog # CI1073) at 1/1000 dilution.|Immunofluorescence images stained on HeLa cells: (Left) Cells stained with anti-H3K27ac polyclonal antibody (Catalog # CI1073) at 1/500 dilution. (Middle) Nuclei stained with DAPI. (Right) Merged images of two stains from the left and middle.[/list_product_images]
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