HCELL-300/杂交瘤细胞

Product Description:
WISENT™ HCELL-300, HCELL-400, HCELL-500 products are serum free medias that have been optimized for the growth of a variety of hybridoma cell lines and production of monoclonal antibodies. Traditionally hybridomas were cultured in media containing serum. Use of serum in culture has many disadvantages and risks for antibody production. The result is a trend towards serum free media for production purposes. HCELL is a group of serum free media products that are designed to grow hybridoma cells and are intended as a monoclonal antibody production media.
Instructions For Use:
General culture conditions :
1) 37°C in a humidified atmosphere of 5 - 10% CO₂.
2) Cultures might be grown in T-Flasks with caps loosened or agitated suspension culture in which case pluronic must be used and adequate head space must be provided.
Adaptation Of Cells To HCELL Serum-free Media :
Adaptation will depend on the particular cell line. For some cell lines direct adaptation is possible while others require sequential adaptation. In both cases cells must be used in mid-logarithmic growth phase with high viability.
1) Direct Adaptation
1.Transfer hybridoma cells growing in media containing serum to HCELL medium which has been prewarmed to 37°C. Seeding density should be double the normal seeding density for the particular cell line.
2. Grow cells until viable cell density reaches 1 x 10⁶/mL. Subculture the cells to a cell density of 1 x 105/mL in fresh HCELL medium for 3 passages.
3. If the culture fails to maintain viability over 3 passages, use the sequential adaptation method.
2) Sequential Adaptation
1. Inoculate hybridoma cells at double the normal seeding density in a 75:25 per cent mixture of serum containing media and HCELL.
2. As soon as the culture density reaches 1 x 10⁶ viable cells/mL subculture into a 50:50 per cent mixture of serum containing media and HCELL.
3. As soon as the culture density reaches 1 x 10⁶ viable cells/mL subculture into a 25:75 per cent mixture of serum containing media and HCELL.
4. As soon as the culture density reaches 1 x 10⁶ viable cells/mL subculture into a 100 per cent HCELL media.
FORM: Liquid, Sterile, Ready to use.
SHEIF LIFE: Maximum 18 months.
STORAGE TEMPERATURE: STORE AT 2 TO 8℃.
热线：400-823-3718
网址：www.wisentbioproductschina.com