Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTPO. Anti-Human TPO specific antibody was purified by affinity chromatography employing immobilized hTPO matrix.
Sandwich To detect hTPO by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human TPO (500-P49Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTPO.
To yield one-half maximal inhibition [ND50] of the biological activity of hTPO (50ng/ml), a concentration of 0.06-0.09 µg/ml of this antibody is required.