大鼠白介素6包被ELISA试剂盒Rat IL-6 Platinum ELISA 96 tests

价格 ¥6019
品牌 eBioscience
货号 85-BMS625
产地详询
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数量：
大量
供应商：
详询
检测限：
HRP
检测方法：
酶联免疫吸附检测
应用：
ELISA
适应物种：
Rat
标记物：
NoData
样本：
详询
规格：
96 tests

描述

The rat IL-6 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of rat IL-6.

Interleukin-6 (IL-6) is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis and may play a central role in host defense mechanisms.
IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections. IL-6 is a pleiotropic cytokine produced by a variety of cells. It acts on a wide range of tissues, exerting growth-induction, growth-inhibition, and differentiation respectively, depending on the nature of the target cells.

IL-6 is involved in

- the induction of B-cell differentiation
- the induction of acute phase proteins in liver cells
- growth promotion of myeloma/plasmacytoma/hybridoma cells
- induction of IL-2 and IL-2 receptor expression
- proliferation and differentiation of T cells
- inhibition of cell growth of certain myeloid leukemic cell lines and induction of their differentiation to macrophages
- enhancement of IL-3-induced multipotential colony cell formation in hematopoietic stem cells and induction of maturation of megakaryocytes as a thrombopoietic factor
- induction of mesangial cell growth
- induction of neural differentiation of PC 12 cells and
- induction of keratinocyte growth

The abnormal production of IL-6 was first suggested to be related to polyclonal B-cell activation with autoantibody production in patients with cardiac myxoma. Since then, IL-6 has been suggested to be involved in the pathogenesis of a variety of diseases. Measurement of IL-6 levels in serum and other body fluids thus provides more detailed insights into various pathological situations.

Conjugate
Biotin

Suitable Sample Types
cell culture supernatant, serum

Sample Volume
50 µL

Reported Application
ELISA

Figures

规格

Assay Range:	31.0-2,000 pg/mL
Assay Sensitivity:	12.0 pg/mL
Detection Method:	Colorimetric
Detector Antibody Conjugate:	Biotin
For Use With (Equipment):	Microplate Reader
Former Identifiers:	Platinum, eBioscience
Gene Aliases:	ILg6, Ifnb2
Gene ID:	24498
Gene Name:	interleukin 6
Gene Symbol:	IL6
Immunoassay Kit Format:	Coated ELISA Kit
Interassay CV:	10%
Intraassay CV:	5.0%
Label or Dye:	HRP (Horseradish Peroxidase)
Product Size:	96 tests
Protein Aliases:	IL-6
Protein Name:	Interleukin-6
Sample Type (Specific):	Cell Culture Supernatants, Serum
Sample Volume:	50 µL
Target (Kit Named):	IL-6
Target Species (Named):	Rat
Target Species (Validated):	Rat
UniProt ID:	P20607

内容及储存

Aluminium pouch(es) with a Microplate coated with monoclonal antibody to rat IL-6
Biotin-Conjugate anti-rat IL-6 monoclonal antibody
Streptavidin-HRP
Rat IL-6 Standard lyophilized, 4 ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Adhesive Films

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