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E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.
Rabbit IgG
Lyophilized
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
单克隆
Human,Mouse,Rat
Rabbit
现货
博士德生物
WB,IHC-P,IF
Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat
100ul/200ul
规格: | 100ul | 产品价格: | ¥680.0 |
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规格: | 200ul | 产品价格: | ¥1180.0 |
货号 | PB0046 |
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产品名称 | ANTI-GFAP Antibody |
基因名 | GFAP |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
免疫原 | E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP. |
内容 | 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA |
纯化方式 | Immunogen affinity purified. |
浓度 | Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat |
产品形态 | Lyophilized |
保存条件 | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
背景资料 | Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament(IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What’s more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication. |
研究类别 | 1. Eng LF, Ghirnikar RS, Lee YL (October 2000). "Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000)". Neurochem. Res. 25 (9-10): 1439–51.2. Tardy M, Fages C, Le Prince G, Rolland B, Nunez J (1990). "Regulation of the glial fibrillary acidic protein (GFAP) and of its encoding mRNA in the developing brain and in cultured astrocytes". Adv. Exp. Med. Biol. 265: 41–52.3. Weinstein DE, Shelanski ML, Liem RK (1991). "Suppression by antisense mRNA demonstrates a requirement for the glial fibrillary acidic protein in the formation of stable astrocytic processes in response to neurons". The Journal of Cell Biology 112 (6): 1205–1213. |
Uniprot ID | GFAP: P14136 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
Immunohistochemistry in paraffin section: | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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