Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin.
内容
200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
研究类别
1. Ngan, B.-Y.; Chen-Levy, Z.; Weiss, L. M.; Warnke, R. A.; Cleary, M. L. : Expression in non-Hodgkin's lymphoma of the BCL-2 protein associated with the t(14;18) chromosomal translocation. New Eng. J. Med. 318: 1638-1644, 1988. 2. Hockenbery, D.; Nunez, G.; Milliman, C.; Schreiber, R. D.; Korsmeyer, S. J. : Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]
Lane 1: human 22RV1 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human COLO-320 whole cell lysates,
Lane 4: human PANC-1 whole cell lysates,
Lane 5: human Jurkat whole cell lysates. |IHC(P): Human Intestinal Cancer Tissue|IHC(P): Human Mammary Cancer Tissue[/list_product_images]