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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1516 |
---|---|
产品名称 | Human R-Spondin-1 ELISA Kit |
产品描述 | For quantitative detection of human R-Spondin-1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, S21-A263 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | RSPO1 |
基因全名 | R-spondin 1 |
Uniprot ID | Q2MKA7 |
指标别名 | CRISTIN3 | hRspo1 | R spondin1 | R-spondin-1 | RSPO | Rspo1 | Q2MKA7 |
背景资料 | R-spondin-1 is a secreted protein that in humans is encoded by the Rspo1 gene. It is mapped to chromosome 1p34. This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy- induced adverse effects. Alternative splicing results in multiple transcript variants. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1516-CAP | 96-well plate precoated with anti- human R-Spondin-1 antibody | 1 |
EK1516-ST | Lyophilized recombinant human R-Spondin-1 standard | 10ng/tube×2 |
EK1516-DA | Biotinylated anti- human R-Spondin-1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 102 | 315 | 845 | 98 | 304 | 805 |
Standard deviation | 5.2 | 16.06 | 42.25 | 6.46 | 16.41 | 45.08 |
CV(%) | 5.1% | 5.1% | 5% | 6.6% | 5.4% | 5.6% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.044 | 0.088 | 0.116 | 0.194 | 0.338 | 0.618 | 1.144 | 2.010 |
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