手机验证
发货周期:现货
博士德生物
cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates.
Mouse
96T
货号 | EK0774 |
---|---|
产品名称 | Mouse HAI-2/SPINT2 ELISA Kit |
产品描述 | For quantitative detection of mouse HAI-2 in cell culture supernates, serum, plasma(heparin, EDTA) and cell lysates. |
免疫原 | NSO, R30-K140 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <4pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | SPINT2 |
基因全名 | serine peptidase inhibitor, Kunitz type, 2 |
Uniprot ID | Q9WU03 |
指标别名 | Bikunin, placental | DIAR3 | HAI2 | HAI 2 | HAI-2 | Kop | PB | Placental bikunin | SPINT2 | Q9WU03 |
背景资料 | Kunitz-type protease inhibitor 2 is an enzyme inhibitor that in humans is encoded by the SPINT2 gene. This gene encodes a transmembrane protein with two extracellular Kunitz domains that inhibits a variety of serine proteases. The protein inhibits HGF activator which prevents the formation of active hepatocyte growth factor. And this gene is a putative tumor suppressor, and mutations in this gene result in congenital sodium diarrhea. Multiple transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0774-CAP | 96-well plate precoated with anti- mouse HAI-2 antibody | 1 |
EK0774-ST | Lyophilized recombinant mouse HAI-2 standard | 10ng/tube×2 |
EK0774-DA | Biotinylated anti- mouse HAI-2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 61 | 240 | 837 | 64 | 248 | 772 |
Standard deviation | 4.14 | 13.92 | 46.03 | 5.56 | 17.85 | 55.58 |
CV(%) | 6.8% | 5.8% | 5.5% | 8.7% | 7.2% | 7.2% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.059 | 0.227 | 0.330 | 0.594 | 0.899 | 1.327 | 1.981 | 2.368 |
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