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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1406 |
---|---|
产品名称 | Human COMT ELISA Kit |
产品描述 | For quantitative detection of human COMT in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, G52-P271 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | COMT |
基因全名 | catechol-O-methyltransferase |
Uniprot ID | P21964 |
指标别名 | Catechol O methyltransferase|Catechol O-methyltransferase|COMT|COMT_HUMAN|EC 2.1.1.6 |
背景资料 | Catechol-O-methyltransferase (COMT) is one of several enzymes that degrade catecholamines such as dopamine, epinephrine, and norepinephrine. In humans, catechol-O-methyltransferase protein is encoded by the COMT gene. As the regulation of catecholamines is impaired in a number of medical conditions, several pharmaceutical drugs target COMT to alter its activity and therefore the availability of catecholamines. In the brain, COMT-dependent dopamine degradation is of particular importance in brain regions with low expression of the presynaptic dopamine transporter (DAT), such as the prefrontal cortex. This process is supposed to take place in postsynaptic neurons, as, in general, COMT is located intracellularly in the central nervous system (CNS). |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1406-CAP | 96-well plate precoated with anti- human COMT antibody | 1 |
EK1406-ST | Lyophilized recombinant human COMT standard | 10ng/tube×2 |
EK1406-DA | Biotinylated anti- human COMT antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 71 | 245 | 923 | 65 | 220 | 946 |
Standard deviation | 4.97 | 16.17 | 61.84 | 5.13 | 16.5 | 79.46 |
CV(%) | 7% | 6.6% | 6.7% | 7.9% | 7.5% | 8.4% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.036 | 0.085 | 0.143 | 0.248 | 0.444 | 0.750 | 1.234 | 2.101 |
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