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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1422 |
---|---|
产品名称 | Human CD73/NT5E ELISA Kit |
产品描述 | For quantitative detection of human CD73 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | CHO,W27-K547 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | NT5E |
基因全名 | 5'-nucleotidase, ecto (CD73) |
Uniprot ID | P21589 |
指标别名 | 5' NT|5' nucleotidase (CD73)|5' nucleotidase precursor|5' nucleotidase, ecto|5' nucleotidase, ecto (CD73)|5'-NT|5'-nucleotidase| 5NTD_HUMAN|CD73|CD73 antigen|E5NT|Ecto 5' nucleotidase|Ecto-5'-nucleotidase|eN|eNT|NT|NT5|NT5E|NTE|Purine 5 Prime Nucleotidase |
背景资料 | 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1422-CAP | 96-well plate precoated with anti- human CD73 antibody | 1 |
EK1422-ST | Lyophilized recombinant human CD73 standard | 10ng/tube×2 |
EK1422-DA | Biotinylated anti- human CD73 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 230 | 1941 | 5361 | 222 | 1912 | 4929 |
Standard deviation | 15.41 | 79.58 | 385.99 | 18.64 | 101.33 | 359.81 |
CV(%) | 6.7% | 4.1% | 7.2% | 8.4% | 5.3% | 7.3% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.093 | 0.116 | 0.168 | 0.248 | 0.422 | 0.797 | 1.401 | 2.056 |
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