The antibody was purified from antisera by immunoaffinity chromatography.
Form
Concentrated, Liquid
Tested Application
IF, FCM
Contents
1mg/ml of DyLight 488 conjugated specific antibody; 0.01M PBS, 5mg BSA/ml, 50% glycerol.
Fluorophore
Amax=493nm; Emax=518nm
Suggested Working Concentration
1:100—500; Optimal working dilutions must be determined by end users.
Application Notes
Please centrifuge before use diluted by neutral PBS or TBS. The diluted solution should be used on the same day. Any unused solution should be disposed of.
Storage
At -20℃ for 12 months from the date of receipt. Protected from light.
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[list_product_images]Figure 1.IHC analysis using Anti-PCNA antibody(BM0104) was detected in immersion fixed HELA cell line. Cells were stained using the DyLight 488 Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1126) and counterstained with DAPI (blue).|Figure 2.IF analysis using Anti-PCK antibody(BM0034) detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the DyLight 488 Conjugated AffiniPure Goat Anti-mouse IgG (H+L) Secondary Antibody (green)(Catalog#BA1126) and counterstained with DAPI (blue).|Figure 3.Beta Tubulin was detected in an immunocytochemical section of U20S cells using mouse anti-Beta Tubulin Antigen Affinity purified monoclonal antibody (Catalog # M05613-4) at 1 μg/mL. DyLight?488 Conjugated Goat Anti-Mouse IgG (H+L) secondary antibody (Catalog # BA1126) was used to detect the primary antibody at 10μg/mL.[/list_product_images]