1mg/ml of HRP conjugated specific antibody;0.01M PBS, 50% glycerol.
Suggested Working Concentration
Western Blotting:1:200-400(DAB detection) Western Blotting:1:2000-4000 (Enhanced chemiluminescent detection ) Direct ELISA:1:10000-100000(TMB detection) Immunohistochemistry:1:100-500Optimal working dilutions must be determined by end users.
Application Notes
Please centrifuge before use diluted by neutral PBS or TBS.The diluted solution should be used on the same day. Any unused solution should be disposed of.And Biotin related reagents used in combination.
Storage
At -20℃ for 12 months from the date of receipt. Protected from light.