Protein A Mag Sepharose
Application |
Designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications Used for biomarker reseach |
Matrix |
Paramagnetic, spherical, highly cross-linked agarose particles |
Ligand |
Native protein A |
Binding Capacity |
> 8 mg human IgG/ml gel |
Operating Temp. |
Room temperature |
Storage Conditions |
4 - 8°C, 20% Ethanol, 20% medium slurry |
Stability |
Stable |
Protein A Mag Sepharose
Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. Based on Sepharose with native protein A as ligand.
- Visible and dense Sepharose based magnetic beads with protein A ligand, easy to spot and collect bound target protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target protein in small or large sample volumes (low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target proteins - only low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
Protein A Mag Sepharose are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose with native protein A as ligand. Protein A has high affinity for the Fc region of IgG antibodies from a broad variety of species
There are two protocols available for protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis
Protein A Mag Sepharose combines well-proven enrichment methods with the magnetic beads platform, which is highly suitable for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel