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    PAR Monoclonal Antibody Affinity Purified

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    PAR Monoclonal Antibody Affinity Purified

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    • 商家诚信度 
    • 入驻年限  7年
    • 深圳市南山区西丽街道阳光一路阳光工业区1栋105

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      • 货号:

        4335-AMC-050

      • 宿主:

      • 克隆性:

        多克隆

      • 形态:

        液体

      • 规格:

        50µl

      特异识别2-50单位长度的PAR聚合酶,但不能识别RNA和DNA相关结构、ADP核糖酶单体、NAD+或者其他核酸单体,用于检测核糖基化蛋白。
      适用于Elisa,WB,IHC-in situ和免疫纯化;可以作为Elisa和WB分析PARP-PAR的阳性对照。

       

      More Details about

      Description: An affinity purified rabbit polyclonal antibody raised against poly(ADP-ribose) (PAR) polymer. Trevigen’s anti-PAR polyclonal antibody can be used to detect ribosylated proteins by immunodetection. Trevigen’s PARP treated control protein (cat# 4500-10-P) and PAR polymer (cat# 4336-100-01) may be used as positive controls.
      Physical State: This antibody is an affinity purified IgG fraction in 1X PBS, containing 50% glycerol.
      Immunogen: Poly(ADP-ribose) polymer.
      Specificity: This polyclonal antibody detects free PAR and poly-ribosylated proteins.
      Storage: -20°C (manual defrost freezer).
      Applications: For western and dot blotting, an antibody dilution of 1:1000 is recommended.
      For ELISA a 1:4000 antibody dilution is recommended. Empirical determination of antibody dilutions will be required for optimum results.


      Cell Lysates for Western Blotting:
      To prepare total cell lysates, cells are solubilized in 1X SDS gel sample buffer (63 mM Tris-HCl, pH = 6.8; 10% glycerol; 2% SDS, 2.5% β-mercaptoethanol, and 0.0025% bromophenol blue) at 1 x 107 cells per ml. The extracts are heated in a boiling water bath for 5 minutes, vortexed for 1 minute at the maximum speed and centrifuged at 10,000 x g for 10 minutes at room temperature. Electrophorese on 4-20% Tris-Glycine SDS-PAGE gels.


      Procedure for Immunoblotting using Peroxidase Detection:
      Blotting buffer: 12 mM Tris base, 96 mM Glycine, and 15% MeOH.
      Blocking solution: 5% (w/v) nonfat dry milk in TBS-0.1% Tween.
      Antibody solution: 5% (w/v) nonfat dry milk, in TBS-0.1% Tween.


      Transfer the electrophoresed proteins to a PVDF membrane and incubate the membrane for 1 hour at room temperature in blocking solution.
      Incubate the membrane overnight at 4°C in antibody solution containing a 1:1000 dilution of anti-PAR rabbit polyclonal antibody. Empirical determination of primary antibody concentration will be required for optimal results.
      Wash the membrane at room temperature for 5 minutes with 3 changes of TBS-0.1% Tween. Changing the membrane containers often reduces background.Incubate the membrane at room temperature for 1 hour in antibody solution containing antirabbit conjugated to horseradish peroxidase.
      Empirical determination of secondary antibody concentration will be required for optimal results.
      Wash the membrane for 5 minutes with 4 changes of TBS-0.1% Tween. Develop peroxidase reaction using chemiluminescence.

       

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