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DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG^® M2 Antibody) (Alexa Fluor^® 555 Conjugate) #3768

Applications	Reactivity	Sensitivity	Source
IF-IC	All	Transfected Only	Rabbit

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.

Protocols

3768:

Immunofluorescence

Specificity / Sensitivity

DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG^® M2 Antibody) (Alexa Fluor^® 555 Conjugate) detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma's Anti-FLAG^® antibodies) fused to either the amino or carboxy terminus of targeted proteins. The binding specificity of this antibody is not dependent on the presence of divalent metal cations.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. The antibody was conjugated to Alexa Fluor^® 555 under optimal conditions with an F/P ratio 2-6.

Description

This Cell Signaling Technology antibody was conjugated to Alexa Fluor^® 555 fluorescent dye and tested in-house for immunofluorescence in cells transfected with DYKDDDDK-tagged protein.

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1).

1. Brizzard, B.L. et al. (1994) Biotechniques 16, 730-5.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

Companion Products

• 2368 DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG^® M2 Antibody)

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.Anti-FLAG® is a registered trademark of Sigma-Aldrich.

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For Research Use Only. Not For Use In Diagnostic Procedures.