*HM* CARB LABELING & ANALYSIS KT N-联寡糖糖谱分析试剂盒
The Carbohydrate Labeling & Analysis Chemistry contains the reagents, buffers, and separation
capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from
glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the
reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS
molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent
oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence.
This includes:
• Carbohydrate Separation Buffer, 56 mL
• N-CHO Coated Capillary, (2)
• Labeling Reagents, (1)
- Labeling Dye (APTS), 4 x 5 mg
- Labeling Dye Solvent, 1 mL
- Glucose Ladder Standard, 50 mg
- Quantitation/Mobility Marker (Maltose), 0.18 mg
• APTS-M (monosaccharide-grade), 20 mg
• Carbohydrate Labeling and Analysis Guide, (1)
Carbohydrate Labeling and Analysis Chemistry Components
477623 N-LINKED GEL BUFFER
477601 NCHO COATED CAPILLARY
50 µm ID x 65 cm total length.
501309 LABELING DYE (APTS) KIT, 2X5MG
725898 APTS-M (21MG)
For monosaccharide analysis (21 mg).