Endothelial Cell Growth Supplement (ECGS) is a medium supplement designed for the optimal growth of human primary microvascular endothelial cells in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human microvascular endothelial cells. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of human primary microvascular endothelial cells in vitro. The supplement is designed as an additive for endothelial cell medium (ECM, Cat. No. 1001) and should be used in conjunction with that medium.
Components ECGS is packaged in the quantity of supplement suited for a 500 ml bottle of endothelial cell medium. When a 500 ml bottle of endothelial cell medium is supplemented with ECGS, the final concentrations of the supplement components per milliliter will be BSA 10 μg, apo-transferrin 10 μg, insulin 5 μg, EGF 10 ng, FGF-2 2 ng, VEGF 2 ng, IGF-I 2 ng, hydrocortisone 1 μg and retinoic acid 10-7 M.
Product Use ECGS is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage Store the ECGS at -20oC before adding to endothelial cell medium.